VIDEO - Membrane Proteins Workshop - Membrane protein purification and application in drug discovery with Arjan Snijder
VIDEO - Membrane Proteins Workshop - Membrane protein purification and application in drug discovery with Arjan Snijder
Speaker: Arjan Snijder, Astra Zeneca
This lecture was part of the workshop, Structural Resolution of Membrane Proteins: From Expression to Sample, organised in May 2021 by the LINXS working group Membrane Proteins, which fall under the Integrative Structural Biology theme.
Abstract
In the presentation I will discuss our capabilities, processes and methods for membrane protein expression and purification, including our recently developed teabag purification method. The teabag method allows rapid purification of membrane proteins with reduced hands-on and processing time. Application of membrane proteins in drug discovery will be illustrated by our work on the protease-activated receptor 2, where extensive protein engineering allowed DNA-encoded library screening on purified receptor, establishment of biophysical assays, and structure assisted drug design.